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KMID : 0895820140240020051
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Journal of Oriental Rehabilitation Medicine
2014 Volume.24 No. 2 p.51 ~ p.64
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Effects of Keonbodan (健步丹) on MIA-Induced Osteoarthritis in Rat
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Lee Eun-Jung
Oh Min-Seok
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Abstract
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Objectives : This study was carried out to know the effects of Keonbodan (hereinafter re-ferred to KBD) in osteoarthritis induced by Monosodium iodoacetate(hereinafter referred to MIA) on Wistar rat.
Methods : Osteoarthritis was induced by injection of MIA into left knee joint cavities of rat. Osteoarthritis rats were divided into 4 groups (normal (n=6), control (n=6), indomethacin (n=6), KBD (n=6) group). The control group was administered normal saline and in-domethacin group was administered indomethacin (2 mg/kg). And the KBD group was ad-ministered KBD (142 mg/kg). Each groups were administered by orally for 4 weeks. This experiment were carried out in vivo. In vivo, at the end of the experiment (5 weeks after MIA injection), effects on hepatotoxicity and nephrotoxicity, cytokines in serum, arachidonic acid, osteocalcin, MMP-9, TIMP-1 and cartilage volume were evaluated. And histopatho-logical examinations on the articular structures of knee joints were performed.
Results : 1. In weight-bearing measurement, level of weight was increased. 2. In order to hepatotoxicity and nephrotoxicity, ALT, AST, BUN and creatinine were tested. And there were no significant changes. 3. In serum, levels of TNF-α, IL-1β were significantly decreased. IL-6 was insignificantly decreased. 4. In serum, level of MMP-9 and TIMP-1 was decreased. 5. In serum, level of LTB4, PGE2 and osteocalcin was decreased. 6. In μCT-ar-thrography, the cartilage volume was greater than that of the control group. 7. The joint damage induced by osteoarthritis was lesser than the control group in histopathologic ob-servation (H&E, Safranin-O staining).
Conclusions : These results demonstrated that KBD suppressed the osteoarthritis- in-ducing effects of MIA in rat. And further studies are required to find out more effective sub-stance and anti-osteoarthritic mechanism in the future.
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KEYWORD
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Keonbodan, KBD, Osteoarthritis, Monosodium iodoacetate, MIA, Inflam-matory cytokine, MMP-9, TIMP-1
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